Coding

Part:BBa_K2201263:Design

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) with an amber stop codon at amino acid po


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 809
    Illegal BamHI site found at 1490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amber stop codon (TAG) is inserted via site directed mutagenesis.


Source

BBa_K1465202 (RuBisCo).

References